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The cell medium was replaced again, and 10 L of MTT (5 mg/mL) was added and incubated for 4 h to generate the MTT-formazan
The data are presented as mean SD from three independent experiments
More importantly, autophagy deficiency leads to accumulation of p62, a multifunctional cargo receptor that can sequester KEAP1 and stabilize NRF2, resulting in NRF2 activation [10]
The compound dramatically reduces liver fat, improves insulin sensitivity, and enhances metabolic markers across multiple domains
This susceptibility stems from reduced basal GSH levels caused by impaired plasma membrane expression of SLC7A11
