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But the results have been largely inconclusive, in part because the data often come from observational studies, which are less reliable and can be muddied by other factors

In the following sections, we describe how the use of mimetic systems can provide such a controlled environment for the analysis of PFPs (Section 5) and how the advent of single-molecule techniques has overcome the previous limitations in the investigation of pore formation by providing information at the level of the individual protein complex (Section 6)

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