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Zarghami M
Measurement of hepatic HNE content The hepatic content of HNE after 14 days of feeding was determined using enzyme-linked immunosorbent assay (ELISA) commercial kits (Shanghai Enzyme-linked Biotechnology, Shanghai, China)
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Cell cultures (150 cm 2 at 80% confluence) were trypsinized and collected with lysate buffer (trichloroacetic acid 0.1 g/L, hydrochloric acid 0.774 M, mercaptoacetic acid 30 mM) to give 100 mg of cells for 400 L buffer
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