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The PCR programme used consisted of a first denaturation step at 98 C for 3 min followed by 20 cycles of: (a) 1 min at 98 C, (b) 45 s at 56 C, and (c) 7 min at 72 C

Melatonin Res 3:3242 Back K, Tan DX, Reiter RJ (2021) Strategies to generate melatonin-enriched transgenic rice to respond to the adverse effects on rice production potentially caused by global warming

For both treatments, frequent sessions provide optimal results

The homogenates were centrifuged at 4 C for 20 min at 12,000 g , and the resulting supernatants were used for MDA analysis

Moreover, studies have identified multiple glutamine metabolic pathways in AML cells