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They were then washed with 0.15 M HEPES three times for 5 min, postfixed with 1% OsO4 (EMS, Hatfield, USA) in 0.1 M Na-cacodylate-buffer (Merck, Darmstadt, Germany) at 4 C for 1 h
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doi: 10.1183/13993003.01836-2020 [DOI] [PMC free article] [PubMed] [Google Scholar] 145.Sykes A, Edwards MR, Macintyre J, et al