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Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h
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We also confirmed that any contraction or expansion caused by adding sugar to a waterethanol mix is negligible for ABV prediction purposes
Cell scattering, which is the hallmark of an HGF/c-Met interaction, leads to a loss of cell adhesion properties that allow cells to migrate
Testosterone replacement for aging men: indications, benefits, and safe clinical management [39:45]
