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Lysates were denatured by boiling in Nupage loading Buffer (Cat#NP0007) and Sample Reducing agent (Cat#NP0004) (10 min, 90 C) and resolved in Nupage 412% gels (Cat#NP0321BOX) using MOPS SDS running buffer (Cat#NP0001)

twice-daily injections typical for injectable protocols No refrigeration needed (capsules stable at room temperature) Convenient for travel and busy schedules Dietary supplement status (no prescription required) Injectable Characteristics: Potentially higher bioavailability reaching systemic circulation (comparative human data does not exist) Established user base with extensive anecdotal reports Ability to perform local injection near injury sites Dosing protocols from practitioner guidance more available Consideration: Oral BPC-157 suits needle-averse beginners prioritizing convenience

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Detailed information on the chemicals, including stock and final working concentrations, is provided in Supplementary Table 3

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