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The pooled sample was purified using a high-capacity reverse phase cartridge (Oasis HLB, Waters) and the eluate was fractionated using high pH reverse phase spin columns (Pierce) according to manufacturer specifications, yielding 8 fractions
Moreover, cbs-1 overexpression in the nematode intestine or body wall muscles extends lifespan at standard (20 C) and high (25 C) rearing temperatures [137]
