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[Abstract] Naunyn Schmiedebergs Arch Pharmacol

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Indirect assay for effect of Gid12 on Gid4 dissociation from GID SR4 0.5 M purified GID SR4 and Gid12 bound GID SR4 complexes with 3FLAG tagged at the C-terminus of Gid8 were incubated with 0, 0.5, 1, 1.5, 2, 2.5 M of 2Strep-Gid4, or 4 M of 2Strep-Gid4 mut (Gid4 residues 1-358, lacking the C-terminal four residues that are required to bind Gid5 and incorporate into GID SR4 ) 21 , respectively

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