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There is no doubt that there is a place for both post transplant cyclophosphamide and CD3+TCR alpha beta depletion depending on center expertise
doi: 10.1007/S11356-017-0555-4 2 AbdelmegeidM
doi: 10.1038/sj.npp.1300699 [DOI] [PubMed] [Google Scholar] 114.Halliwell B
Centrifuge for 10 min (12000 rpm, 4C), take 200 L of the lower chloroform layer into a new centrifuge tube
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