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Figure 2 3.1.1 Preclinical foundations and mechanisms Preclinical studies in animal models have established the foundational rationale for this approach

It is indicated that ALA could enhance the intracellular synthesis of GSH by increasing the availability of its precursor cysteine (40)

When HeLa cells are treated with Ero1 inhibitor (EN460), ER oxidative protein folding is impaired, reducing H 2 O 2 signaling and inhibiting Nrf2 translocation to the nucleus, resulting in a decrease in GPX8, resulting in increased ER calcium content and signaling (Granatiero et al., 2019)

In Arabidopsis, RbohE gene is expressed during micropore development in the anther tapetal layer

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