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A 2000 study involving obese Zucker rats concluded that Fragment 176-191 did not negatively impact insulin sensitivity [11], while a 2001 study involving obese mice found that insulin secretion was unaffected [12]
Therefore, these natural products are considered effective alternatives to antibiotics, primarily to mitigate residual effects in animal products such as milk, meat, and eggs [18]

Vitamin B12 was quantified via electrochemiluminescence using the Roche Diagnostics vitamin B12 II assay performed on the Roche Diagnostics e602 (Calgary Laboratory Services)
