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The tumor bed was defined as the sum of 1) residual viable tumor (RVT), 2) necrosis, and 3) regression bed characterized by the presence of dense tumor-infiltrating lymphocytes (TIL) infiltrates, tertiary lymphoid structure (TLS), features of cell death (keratinous debris and giant cells), and tissue repair (neovascularization, fibrosis)

Study procedure During the study, the appropriate temperature was maintained between 20-24 C, with relative humidity of 40-70% and fluorescent lighting for 12 hours a day