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Despite this, substitution of a polar uncharged side chain (Ser or Thr, -CH 2 OH or -CH 2 (OH)CH 3 ) with a polar negatively charged (Asp, -CH 2 COOH) side chain mimicking phosphorylation is still sufficient to disrupt Golgi localization and fragmentation, implying the 3D mutant likely repulses membranes