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A 20 l aliquot of each pre-cultured sample was mixed with 180 l of SCHisLeuUra+Ade medium and transferred to a flat-bottom transparent 96-well plate (Greiner Bio-One, no
If your protocol requires you to store it for many months after reconstitution, a better (though more advanced) method is to aliquot itdivide the full solution into multiple smaller sterile tubes and freeze them all at once
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