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Gastric Emptying Modulation Dual Pathway Effect Both components contribute to delayed gastric emptying through complementary mechanisms[5]: GLP3 delays gastric emptying primarily through GLP-1R and glucagon receptor pathways Cagrilintide slows gastric transit through amylin and calcitonin receptor activation Combined effects produce more sustained gastric delay than single agents Prolonged nutrient exposure in the small intestine enhances incretin release This dual-pathway gastric slowing mechanism may explain enhanced satiety and reduced caloric intake observed in combination studies

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The copy numbers of integrated genes were verified using real-time PCR (primers GshF-RT1/GshF-RT2, Additional file 1: Table S7) and calculated by a comparative C T method [27]

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