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First, the baseline absorbance was subtracted (T 0 which preceded the addition of the reaction mix) and then these corrected absorbance values at T 1 and T 2 were used to calculate A 405nm = A 2 A 1

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This indicates a strong need for better understanding of the tumor response to the treatment to overcome treatment resistance and reduce tumor progression, as well as to identify therapeutic selectivity, increasing the treatment sensitivity of the tumor while reducing adverse effects in normal tissue [1]

Although MALDI-TOF-MS methods are applied to short oligonucleotide sequencing, the low fragmentation efficiency during ISD or PSD requires the acquisition of numerous spectra, thereby limiting throughput