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Furthermore, measurements of OD 600 showed that deletion of ERD1 only had a minor impact on final cell density, showing OD values of 10.8 for erd1 and 13.2 for RF07, while deletion of PMR1 had a drastic effect on final cell density, reducing the OD to 5.4, a 60% decrease over RF07 (Supplementary Figure S2)

However, as this yolk granule/lysosomal defect gets more severe in the absence of CUP-5, it indirectly impacts the trafficking of some gut granule proteins

This suggests that NHR-80 may regulate other (lipid biology) genes in this context

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People with TMAU may develop depression and anxiety