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When calculating the SNI, the following circumstances may be encountered: new hospitals (for example, hospitals that begin participation in Medicare program after the available audited cost report data), hospital mergers, hospitals with multiple cost reports and/or cost reporting periods that are shorter or longer than 365 days, cost reporting periods that span fiscal years, and potentially aberrant data

84 Importantly, next-generation sequencing and methylation assays have identified key genetic alterations in ctDNA, including mutations in genes such as p53 , EGFR , MET , PIK3CA , and NOTCH1 , highlighting the feasibility of liquid biopsies in molecular profiling and personalized therapy selection