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Notably, these effective concentrations were 16,000-80,000,000 times lower than concentrations of melatonin required for comparable effects, suggesting highly potent regulatory mechanisms rather than simple antioxidant activity
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Per-dose protocol is summarized in the At a Glance above
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A total of 150 ng of RNA from each sample was used in RT-PCRs performed in a total volume of 25 L using the QIAGEN OneStep RT-PCR Kit (Qiagen, Venlo, The Netherlands)
