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Duzniewska et al., 2002

[DOI] [PubMed] [Google Scholar] 11.Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM

After 72 h, the cells were harvested and lysed with 2.0 mL of 1.0% Triton X-100 in TBS (~10 10 6 cells/mL)

The finely tuned regulation of adrenal hormones can be disrupted by oxidative stress, which is caused by an imbalance between reactive oxygen species and the bodys antioxidant defense mechanisms (2)

The disease is spontaneous, is associated with a relatively low frequency within the colony and lesions that wax and wane, making it an interesting observation but not a model that is readily amenable to experimental manipulation