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[DOI] [PMC free article] [PubMed] [Google Scholar] 193.Kienesberger K, Pordes AG, Vlk TG, Hofbauer R

Tissue sections that were 4 m thick were sliced from the paraffin blocks using a microtome (Leica RM2245, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin for histopathological evaluations

Oropharyngeal swabs were performed daily between D1 and D6 and immediately stored at 80 C for further total RNA extraction

Cell Cultures OLN-93 cells, a cell line established from spontaneously transformed rat brain glial cultures [46], were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA), penicillin, and streptomycin at 37 C