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At least 100 comet images from each slide were analyzed using the CASP software ( Western Blotting Cells were lysed in modified radioimmunoprecipitation assay (RIPA) containing 50 mm Tris-HCl (pH 7.4), 150 mm NaCl, 1% Nonidet P-40, 0.1% SDS, 0.5% sodium deoxycholate, 1 mm EDTA, and proteinase inhibitor mixtures

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