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Lysates were sonicated and centrifuged for 15 min at 15,000 g

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Study endpoints include epithelial-barrier function, tight-junction protein expression, inflammatory signaling, and mucosal tissue remodeling in models of intestinal inflammation, increased permeability, and chemically induced gastrointestinal injury

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Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines