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Biotransformations observed in human hepatocytes include hydroxylation, N -glucuronidation, and secondary O -glucuronidation following either O-demethylation or hydroxylation [2]

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Pemetrexed, a clinically applied multi-target antifolate cytotoxic chemotherapeutic drug, has been effective in treating breast cancer and metastatic breast cancer patients previously treated with anthracycline or taxane, with lower toxicity levels (Zhou et al., 2015)

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Subsequently, the proportion of tritium water increased and that of [ 3 H]proline decreased gradually with time

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