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11 When HSP90 function is inhibited, the AR protein misfolds due to the loss of chaperone protection and is eventually degraded by the ubiquitinproteasome system, thereby blocking the AR-dependent transcriptional program

Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

FIGURE 4 In addition to the iron death we mentioned above, GPX4 also plays an important role in the process of sexual maturation in males