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doi: 10.4103/1008-682X.122203
In all cell experiments, the perfusion temperature was maintained at 32C 2C using Heat Wave 30 (Dagan Corporation, United States) perfusion systems
The pellets were gently resuspended in an equal volume of ice-cold MITO buffer (220 mM mannitol, 75 mM sucrose, 10 mM HEPES-KOH [pH 7.4])
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