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[DOI] [PMC free article] [PubMed] [Google Scholar] Suzuki T, Li W, Zhang Q, Novak EK, Sviderskaya EV, Wilson A, Bennett DC, Roe BA, Swank RT, Spritz RA

However, prior studies have shown that conducting 3 H-leucine incubations in the light can increase uptake rates 1.5-fold compared to dark incubations 37 , indicating that results from dark incubation requires careful interpretation and further evaluation

At the end of this step, 20 L of 200 mM DTT was added before digestion with 50 L trypsin overnight, at 37C
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