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Electrodes were positioned in the DI lead and connected by flexible cables to a differential AC amplifier (model 1700, A-M Systems, USA), with signal low-pass filtered at 500 Hz and digitized at 1 kHz by a 16-bit A/D converter (Minidigi 1-D, Axon Instruments, USA) using Axoscope 9.0 software (Axon Instruments, USA)

10.1002/j.1939-4640.1998.tb02057.x 77 RuehleM

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Choline synthesis takes place in the liver and kidney and involves the conversion of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) via the actions of the methyltransferase, phosphatidylethanolamine N -methyltransferase (PEMT)