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With the sub-toxic dose of 0.1 mM, the low gradient of 40 is temporary, with cells rapidly starting to recover the Tpx1-driven gradient of 300, while with the toxic dose of 0.5 mM the gradient collapse is much more sustained and does not recover even after 50 min

D.FazoJ.et al

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At the end of the experiments, the mice were euthanized by injection with an overdose of sodium pentobarbital (200 mg/kg i.p.), the blood samples were centrifuged and the supernatant was collected for further use

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