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The 5mC and 5hmC immunoprecipitation signals were determined using gene-specific primers (Supplementary Table 1)

From there: Volume to draw (mL) = Desired dose (mg) divided by Concentration (mg/mL) And to convert mL to insulin syringe units: Units = Volume (mL) multiplied by 100 Three formulas

Luminescence was measured using a Victor3 platereader equipped with an infrared emission filter every 30 seconds for 10 minutes

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