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Following harvest, washing, and resuspension in PBS with 1% BSA, lipid peroxidation levels were evaluated and quantified through flow cytometry (FACSCanto TM II, BD Biosciences)

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-Glutamylcysteine is a GPx1 cofactor that detoxifies H 2 O 2 To decipher how -glutamylcysteine detoxified ROS but otherwise avoiding the influence of cytosolic GCL, we expressed a silent mutant mito GCL form ( mito GCL (mut)) refractory to the action of a small hairpin RNA against GCL (shGCL) 5 , in HEK293T cells (Fig

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