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Specifically, PROTAC 82 degraded ATR with a DC 50 value of 22.9 nM and a D max value of 76% in MV-4-11 cells and showed the ability to inhibit cell proliferation of several AML cells (IC 50 values ranging from 0.111.3 M)

Inactivation of GPX4 in cells triggers the accumulation of lipid peroxides, leading to ferroptosis 28

Liposomes and soluble recombinant proteins were resuspended to a final concentration of 1 mM total lipids and 5 M recombinant proteins in either PBS pH 7.5 or 150 mM NaCl, 50 mM HEPES pH 7.5 as described 24,64

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In vitro glutathione deficiency alters vitamin D metabolism genes and VDR in mouse hepatocytes To rule out the non-specific effect of HFD conditions and other confounding variables in animals, we carried out in vitro experiments to determine the direct impact of glutathione deficiency status on VD metabolism genes in hepatocytes