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doi: 10.1016/S0140-6736(14)61059-X

Skeletal Muscle Energy Metabolism in Obesity

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Image transformation for size distribution In contrast to the capillary setup where a single vesicle is followed with the motor stage and camera of the microscope, in the chamber setup the entire chamber is scanned

thus, after exceeding this concentration of edelfosine, excess NBD-Sph can participate in micelle formation, which is most likely what is observed as an intensity increase after the addition of 1 M edelfosine to 1 M NBD-Sph (Fig S2C)
