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The results revealed that the levels of pro-inflammatory cytokines IL-6, IL-12, and TNF- secreted by cells treated with NP4 were 1.45, 5, and 1.78 times higher than those secreted by cells treated with NP2, respectively, further confirming the activation of macrophages

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Dysbiosis destroys the integrity of intestinal barriers, thus making the mucosa permeable to pathological organisms and leading to cell death among small intestinal cells such as Paneth and goblet cells (70, 71)

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20ppm, 40ppm, 60ppm, 80ppm, lOOppm were prepared in phosphate buffer pH 7.4 and the absorbance was measured at 227nm
