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Proteins were separated via SDS-PAGE and then transferred to PVDF membrane (Millipore, Billerica, MA, USA)
In cardiomyocytes, NO plays a key regulatory role in intracellular calcium handling, mitochondrial respiration, and redox balance 38,39,40
Furthermore, infiltrating macrophages, cytokine production and inflammatory pathways are systemically increased during aging (inflammaging) also at the skeletal muscle level with a concomitant alteration of redox-mediated signaling pathways and muscle loss [92,93,94]
Young CL, Britton ZT, Robinson AS: Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications
Adequate thyroid hormone levels promote a healthy heart rate and contribute to overall cardiovascular health
