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These alterations are thought to impact gut barrier integrity and immune function, both of which are implicated in CFS/ME pathophysiology

+ The injection uses a small-gauge needle into the deltoid or gluteal muscle

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In this review article, the authors have provided a detailed overview of these strategies

ATP-based luminescence assays, such as the CellTiter-Glo 2.0 Assay, are the preferred platform for HTS drug screening because they offer the highest sensitivity (detecting 15100 cells/well), the simplest protocol (single reagent addition, 10-minute read time), and the lowest coefficient of variation across plate formats