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After washing, intracellular staining was performed using anti-FoxP3-AF647, anti-RORt-BV421, and anti-IL-17-BV510 for 20 minutes at RT in the dark

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The demyelination observed in this study in the cuprizone pellet group is in the early phase at 3 weeks, and a longer period of exposure to cuprizone of 5 weeks may have resulted in a more severe demyelinating effect 39

Effects of intravenously administered vitamin B12 on sleep in the rat
GHK-Cu stimulates the formation of new capillaries, which improves tissue perfusion
