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Through downregulation of pro-ferroptotic protein Acyl-CoA synthetase long chain family member 4 (ACSL4) and upregulation of anti-ferroptotic factors such as glutathione peroxidase 4 (GPX4), they restore cellular antioxidant balance (39, 40)

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Yet, glutathione is added to the elution buffer in relatively high concentrations (~10 mM)

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