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Regardless of its origin, vitamin D undergoes two steps: first, in the liver, it is hydroxylated by 25-hydroxylase (CYP2R1) to form 25-hydroxyvitamin D [25(OH)D], and subsequently, in the kidney, it is hydroxylated by 1-hydroxylase (CYP27B1) to generate the biologically active form, 1,25-dihydroxyvitamin D [1,25(OH)D] (6)