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Vaginal Tightening procedures can reverse these effects by tightening and firming the vaginal canal, restoring it to a more youthful state, and improving overall vaginal health

These limitations do not invalidate preclinical findings but emphasize the need for human clinical trials with appropriate sample sizes, standardized protocols, and validated outcome measures before establishing clinical efficacy in human patients

Flexibility improvements and reduced general stiffness become apparent during weeks two through four for most individuals

Polymerase chain reaction (PCR) on a T100 thermal cycler (Bio-Rad Laboratories, Hercules, California, USA) was used to amplify the fragment of 198-bp with the following primers: 5-TGA AGG AGA AGG TGT CTG CGG GA-3 (forward) and 5-AGG ACG GTG CGG TGA GAG TG-3 (reverse)

Published preclinical studies used intraperitoneal injection in rodents, which bypasses the subcutaneous absorption variability human protocols face