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In aged mice testes, FOXO4 was predominantly expressed in cytoplasm and showed nuclear localization in some Leydig cells (Figure 6A)

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Culbreth M, Aschner M (2016) Dysregulation of glutamate cycling mediates methylmercury-induced neurotoxicity
This was incubated for 1 min at room temperature to elute RNA and then centrifuged at 12000 x g for 2 mins