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For general peptide preparation, see the peptide reconstitution guide and reconstitution calculator

Ma KS, Wang LT, Sasamoto N, Wang YH, Wei JC, Einarsson JI, et al
doi: 10.1007/s10238-013-0250-9 474 WangSHuaYWubYLiXChiGChenYet al

Spinal Cord Volume Presentation Proportional with the Change in the Spinal Cord Surface Area For the spinal cord volume/surface area correlation, we used the method previously described in [38,39,40,41,42,43,44,45,46]

coli BL21 (DE3) and purified using Ni-NTA Superflow agarose (QIAGEN) and glutathione Sepharose 4B (GE Healthcare) according to the manufacturers instructions