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Step 4: Swirl (Don't Shake) Gently swirl the vial in your palm until the solution is fully clear

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Then, AR/Ad cells were incubated at 37 C for 45 min without CO 2 in seahorse assay medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, while the lactate-treated cells were incubated at 37 C for 45 min without CO 2 in a substrate-limited medium containing 0.5 mM glucose, 0.5 mM L-glutamine, and 0.2 mM sodium pyruvate

doi:10.1016/0304-4157(84)90005-4 ^ Van Dorp D.A., Beerthuis R.K., Nugteren D.H

This patient's prominent voltage criteria for hypertrophy and laterally displaced point of maximal impulse make a primary conduction system disease unlikely