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Then, the strains were resuspended in TSB to adjust the OD 600 to 0.91.0, which was determined through multiple trials to be the most effective concentration for successful plasmid uptake during transformation in Av

TB-500 dosage: Initial loading phase of 4 to 8 mg per week for the first 4 to 6 weeks, typically split into 2 to 3 injections per week

Materials and methods Reagents and antibodies Glutathione (GSH), citric acid, iron trichloride hexahydrate (FeCl 3 6H 2 O), and Safranin O-Fast Green were acquired from SigmaAldrich (China)

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