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However, it has become clear that the most widely used enzyme-linked immunosorbent assays (ELISA) do not detect zonulin but complement C3 and possibly properdin, a related molecule from the same family with unclear functional effects (73, 74)

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showed that during intestinal inflammation, the GVB is disrupted, thus determining a state of bacterial product translocation and systemic inflammation, eventually leading to the propagation of inflammation to the brain, while the vascular barrier in the choroid plexus shuts down, helping protect the brain from inflammation but also potentially impairing some brain functions and causing anxiety [42]

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