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Flowcytometric analysis of reactive oxygen species (ROS) Intracellular ROS was measured by DCFH-DA as per the directions of the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA) by using BD FACS VERSE with excitation at 488 nm (blue filter)
Furthermore, these noble NPs can act as seeds for protein aggregation
Bursts of ROS are commonly observed within the first few minutes of reperfusion and have been associated with increased propensity for arrhythmias [2] and mechanical dysfunction [1]

The average number of weekly hours spent in caregiving was 35 h/week

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