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After 30 min, 1 h, 3 h, and 6 h of incubation at 37 C, the cells were stained with Calcein AM (Invitrogen, Carlsbad, CA) to detect live cells and then the capillary-like structures were imaged using EVOS FL Auto (Thermo Fisher)
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Functional monocytic myeloid-derived suppressor cells increase in blood but not airways and predict COVID-19 severity
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