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Although the use of targeted metabolite tracing, such as deuterium tracing of NADPH [7], is successful and remains the gold standard in the field, this approach requires mass-spectrometry, which may not be accessible to many laboratories

Duried Alwazeer, Email: [email protected], [email protected]

1 Introduction Ferroptosis is a non-apoptotic form of regulated cell death driven by iron-dependent membrane lipid peroxidation, which compromises plasma membrane permeability and integrity, ultimately leading to membrane rupture and cell death ( Though ferroptosis is a relatively newly discovered form of regulated cell death, ferroptosis has gained considerable attention, and increasing evidence suggested that it plays a critical role in various diseases, including cancer, neurodegenerative diseases, and tissue ischemia injury ( 2 Mechanisms of ferroptosis Mechanistically, cellular lipid peroxidation occurs in three key steps: (1) Initiation, where reactive oxygen species (ROS) abstract a hydrogen atom from PUFAs-containing membrane phospholipids, generating lipid radicals (L )

Methylsulfonylmethane, commonly known as MSM, is a naturally occurring and essential compound found in humans, as well as in green plants and animals, playing a vital role in supporting overall health and vitality