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Description
Concentrate the cleaved unbound protein to a final volume that is 0.5 to 1% of the column volume to be used for the separation using an ultrafiltration device, such as Amicon Ultra (Millipore), according to the manufacturers instructions (see Note 37)
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[DOI] [PMC free article] [PubMed] [Google Scholar] 198.Kayiklik A., Guvenmez O
Mol Pharmacol 58:15701580 [DOI] [PubMed] [Google Scholar] Arttamangkul S, Torrecilla M, Kobayashi K, Okano H, Williams JT
